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    • Home
    • Urgent On Site Testing
    • Oxford House Drug Testing
    • WeAssure Training Courses
      • DOT Collector Training
    • Services We Offer
      • Drug Testing
      • 5 Panel Drug Testing
      • 9 Panel Drug Testing
      • 10 Panel Drug Testing
      • After Hours Drug Testing
      • Breath Alcohol Testing
      • Fingerprint Checks
      • Group Fingerprinting
      • ATF NFA Fingerprinting
      • FINRA Fingerprinting
      • Printscan Fingerprinting
      • Accredited DNA Testing
      • Paternity DNA Testing
      • Legal DNA Testing
      • Informational DNA Testing
      • Vendor Credentialing
      • Mobile Passport Photos
    • Schedule an Appointment
    • Testing Types
      • DOT Drug and Alcohol
      • Mouth Swab Drug Test
      • ETG Test
    • Information Center
      • Types of Fingerprints
      • Drug Testing Basics
      • DOT Medical Marijuana
      • DOT CBD Update
    • About Us
    • Sure Blog
    • Contact Us
    • Visit Our Notary Site!
WeAssure Biometrics And Screening Services

Reliable, Certified, Mobile. WeAssure It.

  • Home
  • Urgent On Site Testing
  • Oxford House Drug Testing
  • WeAssure Training Courses
    • DOT Collector Training
  • Services We Offer
    • Drug Testing
    • 5 Panel Drug Testing
    • 9 Panel Drug Testing
    • 10 Panel Drug Testing
    • After Hours Drug Testing
    • Breath Alcohol Testing
    • Fingerprint Checks
    • Group Fingerprinting
    • ATF NFA Fingerprinting
    • FINRA Fingerprinting
    • Printscan Fingerprinting
    • Accredited DNA Testing
    • Paternity DNA Testing
    • Legal DNA Testing
    • Informational DNA Testing
    • Vendor Credentialing
    • Mobile Passport Photos
  • Schedule an Appointment
  • Testing Types
    • DOT Drug and Alcohol
    • Mouth Swab Drug Test
    • ETG Test
  • Information Center
    • Types of Fingerprints
    • Drug Testing Basics
    • DOT Medical Marijuana
    • DOT CBD Update
  • About Us
  • Sure Blog
  • Contact Us
  • Visit Our Notary Site!

DOT Urine Specimen Collector Qualification Training

Live, Instructor-Led Training for DOT Drug Screen Collectors

Become qualified to perform DOT-regulated urine drug screen collections through practical, instructor-led training based on the requirements of 49 CFR Part 40.


WeAssure Screening Services provides hands-on collector training designed to build both regulatory knowledge and practical collection skills. Training covers the complete DOT collection process, common problem collections, documentation requirements, specimen integrity, and the situations collectors are likely to encounter in the field.

Requirements to Become a DOT Urine Specimen Collector

Under 49 CFR Part 40 §40.33, a collector must successfully complete two components before performing DOT urine specimen collections:

Qualification Training

Collectors must receive instruction covering the DOT collection process, proper completion and transmission of the Federal Custody and Control Form (CCF), problem collections, fatal and correctable flaws, specimen security, employee privacy, and the collector's responsibilities throughout the collection process.

Initial Proficiency Demonstration

After completing qualification training, the collector must demonstrate proficiency by successfully completing five consecutive error-free mock collection scenarios in accordance with DOT Rule 49 CFR Part 40 §40.33.


WeAssure offers qualification training and proficiency demonstrations separately or together as a complete qualification package.

What You Will Learn

Our DOT collector course covers the knowledge & procedures needed to excel

Collector responsibilities under 49 CFR Part 40

Preparing and securing the collection site

Proper donor identification

Completing the Federal Custody and Control Form

Specimen collection and temperature requirements

Split specimen procedures

Maintaining chain of custody

Specimen sealing, security, and shipment

Insufficient specimen procedures

Specimen integrity and suspected tampering

Monitored and directly observed collections

Refusals and problem collections

Fatal and correctable flaws

Correction of collection and documentation errors

Employee privacy and collector conduct

Advanced Documentation Procedures

Administration-Specific Distinctions

Why DOT procedures exist and how to perform them correctly

Training & Qualification Options

Complete Qualification Course

$450.00

DOT Collector Training + Proficiency Demonstration

Participants complete the required class instruction and assessment, followed by five consecutive error-free mock collections.
Upon completion, participants receive a Certificate documenting the training and proficiency requirements of 49 CFR Part 40.

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Qualification Training Only

$250.00

Training covering requirements of 49 CFR Part 40

For individuals who need the required DOT collector qualification training but will complete their proficiency demonstrations with another qualified trainer or monitor.

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Initial Proficiency Demonstration Only

$300.00

Evaluate 5 consecutive error-free mock collections

For individuals who have already completed compliant DOT collector qualification training and need to complete their required proficiency demonstrations.

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Onsite DOT Collector Qualification Training

INCLUDES BOTH QUALIFICATION INSTRUCTION AND REQUIRED PROFICIENCY DEMONSTRATIONS

WeAssure can bring complete DOT collector qualification training directly to employers, clinics, occupational health providers, and organizations training multiple collectors.


Standard onsite training is available for groups of up to 10 participants.


Larger groups may require additional scheduling and customized arrangements.


Onsite training is offered as the complete qualification program only. Qualification-training-only and proficiency-demonstration-only options are available through our classroom training.

ON-SITE PRICING – $650

Each Additional Participant – $250


2 trainees — $900 total
3 trainees — $1,150 total
5 trainees — $1,650 total
10 trainees — $2,900 total

Find out more

Practical Training for Real Collection Situations

Knowing the regulations is only part of becoming a competent collector.

Training includes practical instruction addressing situations collectors may encounter during actual DOT collections, including insufficient specimens, temperature concerns, suspected tampering, refusals, documentation problems, and other collection complications.


 Participants learn how to recognize a problem, determine the appropriate DOT procedure, properly document the situation, and protect the integrity of the collection.

Who our training is for

  • Drug and alcohol testing professionals
  • Occupational health staff
  • Medical and clinical personnel
  • Employer-designated collection personnel
  • Mobile drug testing providers
  • Compliance professionals
  • Individuals entering the drug testing industry
  • Organizations developing internal DOT collection capabilities 

Classroom Training in The Kansas City Area


Classroom training is available in the Kansas City area for individuals and small groups.

Live instruction allows participants to ask questions, work through collection scenarios, practice documentation procedures, and receive direct feedback before completing their formal proficiency demonstrations.

Certificate of Completion


Participants who successfully complete the Complete Qualification Package receive a Certificate of Completion documenting completion of DOT urine specimen collector qualification training and the required Initial Proficiency Demonstration.


Participants completing only the proficiency demonstration receive documentation of successful completion of their five consecutive error-free mock collections.


Collectors are responsible for maintaining their qualification records and providing them when requested by DOT agency representatives, employers, or TPAs using or considering their collection services.

Experience Led Instruction


Training is led by an active drug and alcohol testing professional with hands-on experience conducting DOT and non-DOT collections in the field.


Instruction goes beyond ideal collection scenarios to address the documentation issues, problem collections, unusual circumstances, and practical decisions collectors encounter while performing real testing.


No previous specimen collection experience is required for the complete qualification course.

Maintaining Your Qualification

DOT Urine Specimen Collector Qualification Does Not End After Initial Training.

Under 49 CFR Part 40 §40.33, collectors must complete refresher training at least once every five years to maintain their qualification while error correction training mandatory any time a collector commits a fatal flaw, 


WeAssure also offers both of these requirements to support collectors in maintaining their DOT collection qualification. 

Collector Refresher Training

Collector Refresher Training

Collector Refresher Training

Streamlined five-year refresher training for experienced DOT collectors, reviewing the complete collection process, problem collections, fatal and correctable flaws, collector responsibilities, advanced documentation procedures, regulatory changes, knowledge assessment, and required proficiency demonstrations.

Error Correction Training

Collector Refresher Training

Collector Refresher Training

Focused retraining for collectors who require error correction training following a collection-process error that caused a DOT test to be cancelled.
Error correction training focuses specifically on the subject matter area associated with the error and includes the required proficiency demonstration.

Begin Your DOT Collector Qualification Process with WeAssure


Whether you are entering the drug testing industry, adding DOT collections to your existing services, or qualifying collection personnel for your organization, WeAssure provides live, practical training designed to prepare collectors for real-world DOT collections.
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Call today to get started

Frequently Asked Questions

DOT establishes qualification requirements for collectors under 49 CFR Part 40. Collectors who successfully complete the required qualification training and proficiency demonstrations may perform DOT urine specimen collections.


Initial qualification requires five consecutive error-free mock collections performed under observation and evaluation by an appropriately qualified individual.


Yes. Individuals who have already completed compliant qualification training may schedule the Initial Proficiency Demonstration separately.


DOT collectors must complete refresher training no less frequently than every five years.


Some collection errors can be corrected without cancelling the test. If a collector makes an error during the collection process that causes a DOT test to be cancelled, DOT requires error correction training addressing the area in which the error occurred.


Yes. Complete DOT collector qualification training can be provided onsite for employers, clinics, testing providers, and other organizations with multiple trainees.


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WeAssure Screening Services, Greater Kansas City

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